Biomaterial-tissue interaction

The evaluation of biomaterial-tissue interactions with biological materials, polymers, ceramics, metals and composites as well as functional surfaces is carried out using cell lines and primary cells of various target tissues (including endothelial cells, fibroblasts, osteoblasts). In addition to the evaluation of cell viability, morphological analyses are performed using scanning electron, fluorescence and confocal laser scanning microscopy. Additionally, dynamic cell cultivation in a tissue-specific test system is possible, in particular to approximate the physiological conditions in cardiovascular tissue. Flow cytometry is used to characterize cells after isolation from tissue (e.g. blood) and to assess inflammatory behavior after contact with materials or solutions. The influence on cell adhesion and proliferation of drugs, biologically active substances and particles to be used in drug delivery systems is analyzed using an impedance measurement system with integrated live cell imaging. The same system is also used in wound healing assays.

Within the scope of external assignments, in vitro GLP tests are also carried out to assess, for example, the biocompatibility, endotoxin content or irritation potential of materials and to enable approval for medical application.

In addition, selected materials and implant systems are examined in the course of in vivo studies in various preclinical models.